期刊论文

Optimization of Extraction Process of Prunella vulgaris L. Polysaccharide by Plackett-Burman Design and Response Surface Central Composite Design and Study on Activity

中文

中国中医药信息杂志

1005-5304

2019

26

12

67-74

10.3969/j.issn.1005-5304.2019.12.015

2017YFC1701900:国家重点研发计划 2017JJ1023:湖南省自然科学基金 2017JJ4045:湖南省自然科学基金 17B198:湖南省教育厅科学研究项目 kq1801041:长沙市科技计划 kq1801046:长沙市科技计划 kq1804027:长沙市科技计划

本校为第一机构

目的研究夏枯草多糖制备工艺,初步探讨其体外抗氧化、抗炎活性。方法利用超声波辅助酶法提取技术,以夏枯草多糖得率为指标,采用Plackett-Burman试验、爬坡试验和响应面分析法筛选最优提取组合。通过ABTS+自由基、DPPH自由基清除能力及Fe~(2+)还原能力研究夏枯草多糖抗氧化能力;检测夏枯草多糖对脂多糖诱导的巨噬细胞RAW264.7模型炎症因子的影响。结果夏枯草多糖的最佳制备工艺为:纤维素酶浓度为5.0%,酶解时间为150 min,酶解温度为60 ℃,液料比为45∶1。验证试验最优值为5.48%,与预测值(5.36%)非常接近。夏枯草多糖具有显著的DPPH自由基、ABTS+自由基清除作用及Fe~(2+)还原能力;对脂多糖诱导的巨...

Objective To optimize the extraction process of Prunella vulgaris L. polysaccharides (PVP); To preliminarily discuss its antioxidant and anti-inflammatory activity in vitro. Methods Ultrasonic assisted enzymatic extraction technology was used and the yield of polysaccharide was set as the comprehensive index. Plackett-Burman analysis method, steepest ascent design and central composite design were used to screen optimal extraction combination. The antioxidant capacity of PVP was determined by the method of ABTS+, DPPH free radical scavenging an...