期刊论文

Establishment of Suspension Cell Lines of Leonurus japonicus and Transformation of Exogenous Hydroquinone into Arbutin

中文

中国中医药信息杂志

1005-5304

2019

26

2

80-83

10.3969/j.issn.1005-5304.2019.02.017

ZYBZH-Y-HVN-24:国家中药标准化项目（中药标准行动计划项目） 2014SK3013:湖南省科技厅科技一般项目

本校为第一机构

目的建立益母草悬浮细胞系,并对其转化生成熊果苷的条件进行优化。方法以前期实验优选出的愈伤组织为材料建立悬浮细胞系,加入氢醌并培养一段时间后,测定胞内与胞外熊果苷产量,计算转化率。结果建立的益母草悬浮细胞系分散性良好,通过生长曲线的测定,确定加入氢醌的最佳时期为接种培养的第6日;通过对不同浓度氢醌、不同转化时间的研究,结合熊果苷产生的动力学研究,优选出加入3mmol/L氢醌转化12h的熊果苷总产量及胞内产量最高,氢醌转化率也较高。结论益母草悬浮细胞生长速度快,转化氢醌生成熊果苷的能力较强,有望开发成为新的生物转化体系。

Objective To establish the suspension cell lines of Leonurus japonicus and optimize the conditions for conversion of hydroquinone to arbutin. Methods The suspension cell lines were established based on the callus optimized in the previous study. After hydroquinone was added to it and cultured for a period of time, the intracellular and extracellular arbutin production were determined and the conversion rate was calculated. Results Well distributed suspension cell lines were established. Through the measurement of growth curve, it was determined that the best period for adding hydroquinone was ...