期刊论文

Effects of Modified Baishile Formula on Autophagy Protein of Hippocampus in Post-stroke Depression Rats

中文

中国中医药信息杂志

1005-5304

2019

26

2

52-56

10.3969/j.issn.1005-5304.2019.02.012

81603604:国家自然科学基金 201785:湖南省中医药科研项目 201822:湖南省中医药科研项目 17K072:教育厅项目 2018M632972:中国博士后科学基金面上项目 ZYNK201605:教育部重点实验室基金 2018-2020:湖南中医药大学中医学国一流建设学科 kq1802008:长沙市科技局重点项目

本校为第一机构

目的 观察百事乐加味方对卒中后抑郁(PSD)大鼠神经功能缺损评分、形态学改变及海马自噬蛋白mTOR、Beclin-1、LC-3 Ⅱ表达的影响,探讨PSD神经元细胞自噬机制及中药防治作用.方法 采用大脑中动脉栓塞+慢性不可预见温和应激+孤养法制作PSD模型,分组前进行行为学评分,随机分为假手术组、抑郁组、卒中组、PSD组、阳性药组、百事乐加味方组,各给药组给予相应药物,连续28 d.HE、Nissl染色观察大鼠海马形态;免疫组化检测PSD大鼠海马mTOR、Beclin-1、LC-3 Ⅱ的表达.结果 与假手术组比较,PSD组大鼠神经功能缺损评分明显升高,海马mTOR表达明显降低,Beclin-1、LC-3 Ⅱ表达明显升高(P＜0.01);与PSD组比较,百事...

Objective To observe the effects of modified Baishile Formula on the neurological deficit score and the expressions of mTOR, Beclin-1 and LC-3Ⅱ in hippocampus of post-stroke depression (PSD) rats; To explore the preventive and therapeutic mechanism of TCM for post-stroke depression from neuronal autophagy. Methods The rat model of focal cerebral ischemia was established by the method of middle cerebral artery occlusion (MCAO), and the PSD model was established by combining with chronic unpredictable mild stress (CUMS) and solitary nourishment....