期刊论文

Effects of Jinshui Liujunjian Decoction on Expressions of MUC5AC and Aquaporin5 in Lung Tissue of COPD Airway Mucus Hypersecretion Rats Model

中文

中国中医药信息杂志

1005-5304

2019

26

8

54-59

10.3969/j.issn.1005-5304.2019.08.012

国家自然科学基金（81473564）；

本校为第一机构

目的观察金水六君煎对慢性阻塞性肺疾病(COPD)气道黏液高分泌大鼠肺组织黏蛋白5AC(MUC5AC)、水通道蛋白5(AQP5)表达的影响,探讨其治疗气道黏液高分泌的可能机制。方法24只SD大鼠随机分成正常组、模型组、金水六君煎组和阿奇霉素组,每组6只。采用烟熏加气管内脂多糖滴入制作大鼠COPD气道黏液高分泌模型,成模后给予相应药物干预,连续2周。观察大鼠一般情况,小动物肺功能仪检测肺功能,HE染色观察肺组织病理,PAS染色检测气道杯状细胞数及黏液量,RT-PCR和Western blot分别检测肺组织MUC5AC、AQP5基因与蛋白的表达。结果与正常组比较,模型组大鼠肺组织HE染色符合COPD病理改变,肺功能及AQP5基因与蛋白表...

Objective To observe the effects of Jinshui Liujunjian Decoction on expressions of mucin5AC (MUC5AC) and aquaporin5 (AQP5) of chronic obstructive pulmonary diseases (COPD) airway mucus hypersecretion rats model; To explore possible mechanism of Jinshui Liujunjian Decoction for the treatment of airway mucus hypersecretion. Methods Twenty-four SD rats were randomly divided into normal group, model group, Jinshui Liujunjian Decoction group and azithromycin group, with 6 rats in each group. COPD model was established by the method of cigarette smoking combined with intratracheal injection of LPS. ...