期刊论文

Effect of Xiaoyao Kang'ai Jieyu Prescription on Hippocampal Neuron Postsynaptic Density Protein 95 Expression in Mice with Breast Cancer Related Depression

中文

中国中医药信息杂志

1005-5304

2019

26

9

43-47

10.3969/j.issn.1005-5304.2019.09.011

湖南省自然科学基金青年基金(2017JJ3235) 湖南省教育厅优秀青年资助项目(17B201) 湖南省教育厅创新平台开放基金(16K067)

本校为第一机构

目的 观察逍遥抗癌解郁方对乳腺癌并发抑郁症(BCRD)小鼠海马神经元突触后致密蛋白95(PSD95)的影响,探讨其对海马组织的可能保护机制.方法 腋下注射4T1炎性乳腺癌细胞联合背部皮下注射皮质酮建立BCRD小鼠模型.随机分为模型组、紫杉醇组、紫杉醇+氟西汀组、逍遥抗癌解郁方组、紫杉醇+逍遥抗癌解郁方组,另取未造模BALB/c小鼠作为正常组.采用悬尾实验和强迫游泳实验检测小鼠抑郁样行为,HE染色观察小鼠海马和乳腺肿瘤病理变化,免疫组化染色和Western blot检测小鼠海马PSD95的表达.结果 与正常组比较,模型组小鼠悬尾实验、强迫游泳实验不动时间显著增加(P

Objective To study the effect of regulation of Xiaoyao Kang'ai Jieyu Prescription on hippocampal neuron postsynaptic density protein 95 (PSD95) in mice with breast cancer related depression, and to elucidate its possible mechanism of protect hippocampus. Methods The BCRD mice model was established by axillary injection of 4T1 inflammatory breast cancer cells combined with subcutaneous injection of corticosterone in the back and randomly divided into model group, paclitaxel group, paclitaxel + fluoxetine group, Xiaoyao Kang'ai Jieyu Prescription group, paclitaxel + Xiaoyao Kang'ai Jieyu Prescri...