期刊论文

Study on Freeze-drying Process and Properties of Cantharidin Galactosylated Liposomes

中文

中国中医药信息杂志

1005-5304

2019

26

10

70-75

10.3969/j.issn.1005-5304.2019.10.016

国家自然科学基金面上项目(81573621).

本校为第一机构

目的制备斑蝥素半乳糖化脂质体（Lac-CTD-Lips）冻干粉,并对其理化性质进行考察。方法利用冷冻干燥法制备Lac-CTD-Lips冻干粉,以粒径、外观、复溶时间及复溶后外观为指标,采用单因素试验筛选冻干处方最佳工艺,考察冻干前后脂质体的形态学变化、粒径、p H值及包封率。结果采用内外加法,冻干保护剂的质量浓度为20%,甘露醇-海藻糖比为3∶2,以速冻的方式,于-50℃冻干机冷阱预冻,冷冻干燥24 h,可获得较满意的冻干粉。与冻干前比较,冻干后脂质体形态未发生明显变化;冻干前后脂质体的粒径分别为（211.6±0.05）nm、（233.2±0.12）nm,Zeta电位分别为（0.08±0.01）m V、（-3.30±0.21）m V,包封率分别为...

Objective To prepare lyophilized powder of cantharidin galactosylated liposome(Lac-CTD-Lips);To investigate its physical and chemical properties.Methods Lac-CTD-Lips lyophilized powder was prepared by using freeze-drying method.With particle size,appearance,resolution time and reconstituted appearance as indexes,single factor test was used for the optimization of the process of freeze-drying prescription.The morphological changes,particle size,pH value and encapsulation efficiency of liposomes before and after lyophilization were investigated.R...