期刊论文

Effects of Plasma Containing Invigorating Blood and Dissipating Masses Chinese Medicine on Cell Proliferation-Related Factors and ERK1/2 Signal Transduction Pathway in Rabbit RPE Cell Intervened by PDGF

中文

中国中医药信息杂志

1005-5304

2019

26

2

62-68

10.3969/j.issn.1005-5304.2019.02.014

国家自然科学基金面上项目(81774372) 湖南省自然科学基金(2016JJ6111) 湖南省中医药管理局科研项目(201622) 湖南省研究生科研创新项目(CX2016B377、CX2017B432) 中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018) 长沙市科技计划项目(kc1704005) 湖南省中医五官科学重点学科建设项目(1988年).

本校为第一机构

目的 观察活血散结方对血小板源性生长因子(PDGF)干预下兔视网膜色素上皮(RPE)细胞增殖的影响,探讨其分子机制.方法 以RPE细胞为研究对象,体外进行RPE细胞原代培养及传代,建立PDGF干预下兔RPE细胞增殖模型,并选取PDGF最佳干预浓度.制备活血散结方药物血浆,对兔RPE细胞增殖与毒性的影响进行测定,选取最佳的实验浓度.实验分为空白对照组(DMEM)、正常血浆组、PDGF(10μg/L)组、PDGF(10 μg/L) +AG1296(10 μmol/L)组、PDGF(10 μg/L)+10％药物血浆组、PDGF(10 μg/L)+20％药物血浆组.分别加入相应处理因素干预48 h,Transwell流式细胞仪检测细胞周期变化;Western blot检测兔RPE细胞cyclin D1蛋白、p...

Objective To investigate the effect of plasma containing invigorating blood and dissipating masses Chinese medicine on proliferation of rabbit RPE cells treated with PDGF; To discuss the molecular mechanism. Methods The RPE cells were used as the research object and the primitive culture and subculture of RPE cells were proceeded. Rabbit model of RPE cell proliferation treated with PDGF was established, and the best PDGF intervention concentration was chosen. Plasma containing invigorating blood and dissipating masses Chinese medicine was prepa...