期刊论文

Rapid Determination and Analysis of Honokiol and Total Polysaccharide in Huoxiang Zhengqi Preparations

中文

中国中医药信息杂志

1005-5304

2019

26

5

78-82

10.3969/j.issn.1005-5304.2019.05.017

81270055:国家自然科学基金 81874344:国家自然科学基金 ZYBZH-Y-HUN-24:国家中药标准化项目（中药标准行动计划项目） 2019JJ40220:湖南省自然科学基金 2018M640755:中国博士后科学基金 201811:湖南省中医药科研项目 YF201705:研究生科研创新项目

本校为第一机构

目的建立 HPLC双波长指纹图谱快速测定藿香正气制剂中和厚朴酚含量的方法,建立糖量计法快速测定其总多糖含量的方法,并分析其差异性。方法 HPLC采用XB-C18(HS)色谱柱(4.6 mm×250 mm,;5 μm),乙腈和0.1%磷酸水溶液等度洗脱,流速1.0 mL/min,进样量20 μL,检测波长294、270 nm,柱温30 ℃;糖量计法以50%乙醇为阴性对照;聚类分析重复性样品,对比不同指标间的聚类结果。结果 HPLC测得和厚朴酚在1.40~0.14 mg/mL范围内与峰面积线性关系良好,线性方程为A=12 182 633C-217 418,r²=0.999 5;藿香正气水中和厚朴酚浓度为(0.400 8±0.005 0)mg/mL,藿香正气滴丸中其浓度为(0.487 9±0.008 9)mg/mL。总多糖在...

Objective To establish a method for rapid determination of honokiol in Huoxiang Zhengqi Preparations by HPLC dual-wavelength fingerprint,and to establish a sugar meter for rapid determination of total polysaccharide in Huoxiang Zhengqi Preparations,and to analyze its differences.Methods HPLC was performed on an XB-C18 (HS) column (4.6 mm×250 mm,5 μm),with acetonitrile and 0.1% phosphoric acid aqueous solution for isocratic elution.The flow rate was 1.0 mL/min;the injection volume was 20 μL;the detection wavelength was 294 and 270 nm;the colu...