期刊论文

Effects of Yiqi Huayu Jiedu Prescription on Atophagy Flow of Human Hepatocellular Carcinoma Cell SMCC-7721 Based on PI3K/mTOR Pathway

中文

中国中医药信息杂志

1005-5304

2019

26

10

45-49

10.3969/j.issn.1005-5304.2019.10.011

国家自然科学基金(81603603) 湖南省科技计划项目(2016SK2051) 湖南省教育厅优秀青年项目(14B135) 湖南省中医药管理局重点课题(201803) 湖南中医药大学创新课题(2017CX21).

本校为第一机构

目的观察益气化瘀解毒方对人肝癌细胞SMCC-7721自噬流的影响,初步探讨其可能的作用机制。方法实验分为空白对照组、重楼皂苷Ⅰ组、益气化瘀解毒方组、雷帕霉素组及索拉非尼组,各给药组给予相应药物。体外实验观察人肝癌细胞SMCC-7721自噬流发生情况并检测细胞微管相关蛋白1轻链3(LC-3Ⅱ)、类胰岛素样生长因子2的mRNA结合蛋白质(p62)及磷脂酰肌醇-3-激酶(PI3K)、哺乳动物雷帕霉素靶蛋白(mTOR)的表达。结果重楼皂苷Ⅰ、益气化瘀解毒方及索拉非尼均能阻断人肝癌细胞SMCC-7721自噬流,导致细胞内自噬小体的蓄积,上调LC-3Ⅱ、p62的表达,同时可下调PI3K、mTOR蛋白的表达。结论益气化瘀解毒方可改变人肝...

Objective To observe the effects of Yiqi Huayu Jiedu Prescription on autophagy flow of human hepatocellular carcinoma cell SMCC-7721;To preliminarily discuss its possible mechanism of action.Methods The experiment was divided into blank control group,Chonglou saponin Ⅰ group,Yiqi Huayu Jiedu Prescription group,Rapamycin group and Sorafenib group.Each administration group was given relevant medicine.In vitro experiments were performed to observe the occurrence of autophagy flow in human hepatocellular carcinoma cell SMCC-7721 and to detect the ...