期刊论文

Effects of Modified Naotai Prescription on Inflammatory Pathway of SIRT1/NF-κB in Hypoxia/Reoxygenation Injured Hippocampal Neuron

中文

中国中医药信息杂志

1005-5304

2019

26

3

45-50

10.3969/j.issn.1005-5304.2019.03.011

81603604:国家自然科学基金 81774174:国家自然科学基金 2018M632972:中国博士后科学基金面上项目 201822:湖南省中医药科研项目 09k056:湖南省教育厅项目 201705:湖南中医药大学大学生研究性学习和创新性实验计划

本校为第一机构

目的观察加味脑泰方对大鼠缺氧/复氧损伤海马神经元炎性通路 SIRT1/NF-κB的影响,初步探讨其作用机制。方法原代分离、培养及鉴定 SD大鼠胎鼠海马神经元,采用缺氧 24h、复氧 2h干预构建缺氧/复氧细胞模型。将培养的原代海马神经元随机分为正常组、空白血清对照组、模型组、阳性药药物血清组和加味脑泰方药物血清组,待缺氧 24h后,除正常组和模型组给予等量培养液外,其余各组分别给予相应体积分数 10%药物血清或空白血清,继续复氧共孵育培养 2h;采用 MTT法检测海马神经元细胞活力,高内涵细胞成像分析系统检测海马神经元沉默信息调节蛋白1(SIRT1)、核因子-κB抑制蛋白(IκBα)、核因子-κB(NF-κB)...

Objective To observe the effects of modified Naotai Prescription on inflammatory pathway of SIRT1/NF-κ B in hypoxia/reoxygenation injured hippocampal neurons;To explore the mechanism of action. Methods Hippocampal neurons from SD rats were primitively isolated, cultured and identified. Hypoxia/ reoxygenation (H/R) cell model was established by hypoxia 24 h and reoxygenation 2 h intervention. The cultured cells were randomly divided into normal group, blank serum group, model group, positive medicine serum group and modified Naotai Prescription...