期刊论文

Effects of Huoxue Huayu Mixture on Inflammatory Factors and TLR4/NF-κB Pathway in Rabbit Model of Knee Osteoarthritis

中文

中国中医药信息杂志

1005-5304

2020

27

3

39-42

10.3969/j.issn.1005-5304.201907029

81574004:国家自然科学基金 81874476:国家自然科学基金 19C1420:湖南省教育厅一般项目 2018JCYX04:湖南中医药大学基础医学一流学科开放基金

本校为第一机构

目的 观察活血化瘀合剂对膝骨性关节炎模型兔炎症因子及TLR4/NF-κB通路的影响,探讨其关节保护作用机制.方法 将新西兰兔分为正常组、假手术组、模型组、中药组和阳性药组,每组8只.采用改良的Hulth造模法建立兔膝骨性关节炎模型,成模后中药组给予活血化瘀合剂(2.6 g/kg)灌胃,阳性药组给予硫酸氨基葡萄糖溶液(15 mg/kg)灌胃,正常组、假手术组和模型组给予等体积蒸馏水灌胃,每日2次,连续28 d.末次给药后,切除兔右后膝关节软骨用于组织病理分析,分离血清及关节液,检测肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6含量,Western blot检测关节软骨Toll样受体4(TLR4)、核因子-κB(NF-κB)蛋白表...

Objective To observe the effects of Huoxue Huayu Mixture on inflammatory factors and TLR4/NF-κB pathway in rabbit model of knee osteoarthritis;To discuss its protective mechanism for joints.Methods New Zealand rabbits were divided into normal group, sham-operation group, model group, TCM group and positive medicine group, with 8 rats in each group.A rabbit model of knee osteoarthritis was established by modified Hulth modeling method.After successful modeling, TCM group was given Huoxue Huayu Mixture(2.6 g/kg)for gavage, positive medicine grou...