期刊论文

Effects of Compound Qishao Jiangya Tablets on Expression of P2Y6 Signal Pathway Related Factors of Vascular Endothelial Cells Co-cultured with Smooth Muscle Cells Induced by TNF-α

中文

中国中医药信息杂志

1005-5304

2020

27

11

58-65

10.19879/j.cnki.1005-5304.202004401

国家自然科学基金(81473616) 湖南省中医药管理局重点项目(201910) 湖南省科技厅项目(2018SK51203) 中医内科重大疾病防治及转化教育部重点实验室开放基金(ZYNK201703) 湖南中医药大学研究生校级创新课题(2019CX67)。

本校为第一机构

目的 观察复方七芍降压片对肿瘤坏死因子-α(TNF-α)诱导的与平滑肌细胞共培养的血管内皮细胞中炎症因子与P2Y6、MEK1/2、ERK1/2蛋白表达的影响,探讨其治疗高血压的机制.方法 采用内皮细胞和平滑肌细胞构建共培养体系,利用炎症因子TNF-α 建立高血压炎症微环境.将共培养好的细胞随机分为正常组、模型组、空白血清组、MRS2578组、厄贝沙坦药物血清组、复方七芍药物血清组、MRS2578+复方七芍药物血清组.MRS2578组和MRS2578+复方七芍药物血清组加入10μmol/L MRS2578.正常组和模型组加入等量培养液,余各组加入10％药物血清及空白血清.采用倒置显微镜观察内皮细胞和平滑肌细胞形态;采用ELISA检测细胞...

Objective To observe the effects of compound Qishao Jiangya Tablets on inflammatory factors and P2Y6,MEK1/2,ERK1/2 protein expressions of vascular endothelial cells co-cultured with smooth muscle cells induced by TNF-α;To explore the mechanism of compound Qishao Jiangya Tablets in the treatment of hypertension.Methods Co-culture system was established by vascular endothelial cells and smooth muscle cells,and inflammatory factor TNF-α was used to establish a microenvironment for hypertension inflammation.The cultured cells were randomly divide...