期刊论文

Effects of Paeoniflorin on PINK1-Parkin Mediated Mitophagy in H_2O_2-induced SH-SY5Y Cell Damage

中文

中国中医药信息杂志

1005-5304

2020

27

11

45-51

10.19879/j.cnki.1005-5304.202004479

81774129:国家自然科学基金 2018JJ2296:湖南省自然科学基金 2019JJ50441:湖南省自然科学基金 2018025:湖南省中医药局科研项目 18B246:湖南省教育厅优秀青年科研项目

本校为第一机构

目的观察芍药苷对过氧化氢(H_2O_2)诱导的人神经母细胞瘤细胞(SH-SY5Y)细胞损伤中线粒体自噬的影响,探讨其相关作用机制。方法采用250 μmol/L H_2O_2诱导SH-SY5Y细胞24 h构建细胞损伤模型。实验细胞分为空白组、模型组和芍药苷低、中、高剂量组。采用MTT法检测细胞存活率,JC-1线粒体膜电位荧光探针检测线粒体膜电位,TUNEL染色检测细胞凋亡率,Western blot检测PINK1、Parkin、LC3Ⅱ和p62蛋白的表达。结果与空白组比较,模型组细胞存活率下降,线粒体膜电位下降,细胞凋亡率上升,PINK1、LC3Ⅱ和p62蛋白表达升高,Parkin蛋白表达无明显变化;与模型组比较,芍药苷低、中、高剂量组细胞线粒体膜电位上调,...

Objective To explore the regulatory effects of paeoniflorin on mitophagy in H_2O_2-induced SH-SY5Y cell damage;To discuss its mechanism of action.Methods 250 μmol/L H_2O_2 was used to induce SH-SY5Y for 24 h to construct a cell injury model.The blank group,model group and paeoniflorin low-,medium- and high-dosage groups were involved in the experiment.Methyl thiazolyl tetrazolium assay was used to detect cell survival rate.JC-1 mitochondrial membrane potential fluorescent probe was used to detect mitochondrial membrane potential.TUNEL staining...