期刊论文

Effects of Electroacupuncture on Expressions of BDNF, TrkB and GABAa in Cortex of Rats with Limb Spasm after Stroke

中文

中国中医药信息杂志

1005-5304

2020

27

2

23-27

10.3969/j.issn.1005-5304.201905042

国家自然科学基金(81673886) 湖南省自然科学基金(2018JJ2294) 湖南省中医药科研计划项目(201828) 湖南省研究生科研创新项目(CX2018B488)。

本校为第一机构

目的观察电针对脑卒中肢体痉挛大鼠皮质脑源性神经营养因子(BDNF)、酪氨酸激酶受体B(TrkB)、γ-氨基丁酸(GABA)受体(GABAa)表达的影响,探讨电针治疗脑卒中肢体痉挛的机制。方法将77只SD雄性大鼠随机分为空白组、假手术组各9只及模型储备组59只,运用Zea Longa线栓结合内囊注射 NMDA受体制作脑卒中肢体痉挛大鼠模型。18只成模大鼠随机分为模型组、电针组。电针组取双侧阳陵泉、曲池针刺,每次30 min,每日1次,连续5 d;假手术组和模型组同期只固定不作任何干预,空白组不作任何处理。观察各组大鼠Zea Longa评分,改良Ashworth肌张力量表评分,皮质BDNF、TrkB、GABAa mRNA及蛋白表达。结果与空白组、假手...

Objective To observe the effects of electroacupuncture on the expressions of BDNF, TrkB and GABAa in the cortex of rats with limb spasm after stroke; To explore the mechanism of electroacupuncture in the treatment of limb spasm after stroke. Methods Totally 77 SD male rats were randomly divided into blank group (9 cases), sham-operation group (9 cases), and model reserve group (59 cases). Rat model with limb spasm after stroke was prepared by using Zea Longa suture-occluded and injecting of NMDA receptor in inner capsule. Then, the successfully prepared 18 rats were randomly divided into the m...