期刊论文

Effects of Naotai Formula on Peroxidative Injury of Nerve Cells Caused by Iron Deposition in Brain Tissue of Rats after Intracerebral Hemorrhage

中文

中国中医药信息杂志

1005-5304

2020

27

4

46-51

10.3969/j.issn.1005-5304.201908240

81774174:国家自然科学基金 81774033:国家自然科学基金 201969:湖南省中医药科研项目 2018ZXYJH15:湖南中医药大学中西医结合一流学科开放基金项目

本校为第一机构

目的观察脑泰方对大鼠脑出血后脑组织铁沉积致神经细胞过氧化损伤的影响,探讨其作用机制。方法采用自体血注射法构建SD大鼠脑出血模型,随机分为模型组、脑泰方组、阳性对照组、假手术组,各组均于给药7、14、28 d取材;HE染色观察脑组织病理变化,采用Zea Longa 5级评分法进行神经功能缺失评分,免疫组化检测病灶脑组织铁沉积量、丙二醛(MDA)、谷胱甘肽(GSH)含量,免疫荧光技术检测转铁蛋白(Tf)、转铁蛋白受体(TfR)、谷胱甘肽过氧化物酶4(GPX-4)的表达。结果与假手术组同一时点比较,模型组大鼠脑组织TfR和Tf表达上调,GPX-4表达下调,GSH含量下降,铁沉积量和MDA含量升高,神经功能缺失评分升高,差异均有...

Objective To investigate the effects of Naotai Formula on peroxidation injury of nerve cells caused by iron deposition in brain tissue of rats after intracerebral hemorrhage; To discuss its mechanism of action. Methods Intracerebral hemorrhage model of SD rats was established by autologous blood injection. The rats were randomly divided into model group, Naotai Formula group, positive control group and sham-operation group. The samples were collected in three batches on 7, 14 and 28 d of administration. HE staining was used for histopathological observation; Zea Longa 5 grade scoring was used ...