期刊论文

Effects of Baishile Capsules on Neuronutrition-related Protein Expression of Primary Hippocampal Neurons Exposed to Corticosterone

中文

中国中医药信息杂志

1005-5304

2020

27

7

48-53

10.3969/j.issn.1005-5304.201911523

湖南省自然科学基金青年基金(2018JJ3388) 湖南省教育厅优秀青年项目(18B238) 湖南中医药大学中医学国内一流建设学科开放基金(2018ZYX49)。

本校为第一机构

目的观察百事乐胶囊药物血清对皮质酮暴露大鼠原代海马神经元神经营养相关蛋白表达的影响,探讨其治疗抑郁症的潜在机制。方法采用受孕18 d的SD大鼠胎鼠进行海马神经元原代培养,将神经元随机分为正常组、空白血清组、皮质酮暴露组、氟西汀药物血清组、百事乐胶囊药物血清组。除正常组外,其余各组给予相应干预的同时进行高浓度皮质酮暴露。干预18 h后,光学显微镜下观察神经元生长情况,MTT法检测海马神经元细胞活力,高内涵分析技术检测神经营养相关蛋白的表达。结果与正常组比较,皮质酮暴露组神经元存活率显著降低(P

Objective To observe the effects of Baishile Capsules on neuronutrition-related protein expression of hippocampal neurons under the condition of corticosterone exposure;To explore the potential mechanism of Baishile Capsules on the treatment of depression.Methods The primary culture of hippocampal neurons was performed using SD fetal rats at 18 days of conception, and the neurons were randomly divided into normal group, blank serum group,corticosterone exposure group, fluoxetine drug serum group, and Baishile Capsules serum group.Except for normal group, all of other groups were given interven...