期刊论文

Study on Disease-syndrome Combination Animal Model of Ischemic Stroke Stasis Toxin Syndrome

中文

中国中医药信息杂志

1005-5304

2020

27

9

69-74

10.3969/j.issn.1005-5304.202002282

国家自然科学基金(81874416) 湖南中医药大学研究生创新课题资助项目(2019CX02) 湖南中医药大学中西医结合一流学科开放基金(2018ZXYJH01)。

本校为第一机构

目的探索缺血性中风瘀毒互结证病证结合动物模型制备方法。方法通过肾上腺素/内毒素、角叉菜胶(CA)和角叉菜胶/干酵母菌(CA/Y)3组药物干预,并复合大脑中动脉梗死(MCAO)构建缺血性中风瘀毒互结证大鼠模型。造模后24 h,观察并检测大鼠表征、神经功能评分、脑病理形态学、血液流变学和脑组织促炎因子水平。结果上述3组病证结合组大鼠缺血侧大脑皮质和海马均出现显著神经元坏死和免疫细胞浸润,且CA组和CA/Y组大鼠肢体远端肤色晦黯甚至发黑;其中,CA/Y组神经功能缺损症状和血液流变学改变最显著,低切、高切全血黏度均明显升高(P

Objective To explore the method for establishing the disease-syndrome combination animal model of the ischemic stroke stasis toxin syndrome.Methods The rat model of the ischemic stroke stasis toxin syndrome was established by the treatment of drugs and the surgical method of middle cerebral artery occlusion(MCAO).These drugs were devided into 3 groups,i.e.,adrenaline + endotoxin,carrageenan(CA)and carrageenan + yeast(CA/Y).After that,the surgical model of MCAO was conducted.24 hours after MCAO,the changes of skin color,neurological function sco...