期刊论文

Effects of Acupuncture Combined with Medicine on Expressions of γ-aminobutyric Acid Type a Receptor and Brain-derived Neurotrophic Factor in Hippocampus of Rats with Limb Spasm Caused by Cerebral Stroke

中文

中国中医药信息杂志

1005-5304

2020

27

1

45-50

10.3969/j.issn.1005-5304.201905020

81673886:国家自然科学基金 2018JJ2294:湖南省自然科学基金 201828:湖南省中医药科研项目 CX2018B488:湖南省研究生科研创新项目

本校为第一机构

目的 观察针药结合对脑卒中肢体痉挛大鼠脑组织海马γ-氨基丁酸a型受体(GABAa)和脑源性神经营养因子(BDNF)表达的影响,探究针药结合治疗脑卒中后肢体痉挛的作用机制.方法 采用中动脉线栓结合内囊注射NMDA受体制作脑卒中肢体痉挛大鼠模型.45只实验大鼠随机分为模型组、针药结合组(针刺阳陵泉、曲池十加味芍药甘草汤水煎剂灌胃)、西药组(巴氯芬溶液灌胃)、空白组(不做任何处理)和假手术组(干预同模型组,只分离不结扎不插线).各治疗组大鼠成模后第1日开始干预,连续5d.采用RT-PCR和Western blot分别检测大鼠脑组织海马BDNF、GABAa mRNA和蛋白的表达.结果 与空白组比较,假手术组大鼠神经功能、肌张力...

Objective To analyze the effects of acupuncture combined with medicine on the expressions of γ-aminobutyric acid type a receptor (GABAa) and brain-derived neurotrophic factor (BDNF) in hippocampus of rats with limb spasm caused by cerebral stroke; To explore the mechanism of acupuncture combined with medicine for limb spasm caused by cerebral stroke. Methods A rat model with limb spasm caused by cerebral stroke was made by injecting NMDA receptor into the middle capsule with a middle artery suture. Totally 45 SD rats were randomly divided into...