期刊论文

Effects of Zishen Huoxue Prescription on BDNF/TrkB/PI3K/Akt Signaling Pathways in Vascular Dementia Rats

中文

中国中医药信息杂志

1005-5304

2020

27

10

42-47

10.19879/j.cnki.1005-5304.202005193

国家自然科学基金（81894462）；

本校为第一机构

目的 观察滋肾活血方对血管性痴呆(VD)大鼠BDNF/TrkB/PI3K/Akt信号通路的影响,探讨其可能的作用机制.方法 采用改良的双侧颈总动脉结扎法(2-VO法)建立大鼠VD模型,将大鼠随机分为假手术组、模型组、滋肾活血低剂量组、滋肾活血高剂量组和奥拉西坦组,每组8只.给药28 d后,HE染色观察大鼠海马组织CA1区变化,免疫组化和Western blot分别检测大鼠脑组织p-Akt、Akt、TrkB和BDNF蛋白的表达.结果 HE染色结果:与假手术组比较,模型组大鼠海马组织CA1区损伤较严重,细胞核破裂,胞质浓缩,胞体变小;与模型组比较,滋肾活血低剂量组、滋肾活血高剂量组和奥拉西坦组大鼠海马组织CA1区损伤均明显减轻;免疫组化染色结...

Objective To explore the effects of Zishen Huoxue Prescription on BDNF/TrkB/PI3K/Akt signal pathways in vascular dementia(VD)rats;To discuss its possible mechanism.Methods The modified bilateral common carotid artery ligation method(2-VO method)was used to establish rat VD models, and the rats were randomly divided into sham-operation group, model group, Zishen Huoxue low-, high-dosage groups, and Oxiracetam group, with 8 rats in each group.28 days after administration, HE staining was used to observe the changes in the CA1 region of rat hippocampus.Immunohistochemistry and Western blot were u...