期刊论文

Effects of Electroacupuncture Stimulation of Acupoints of Pericardium Meridian on Nogo-A and NgR1 Expression in Cerebral Ischemia Rats

中文

中国中医药信息杂志

1005-5304

2020

27

10

59-63

10.19879/j.cnki.1005-5304.202004131

81704184:国家自然科学基金 81774421:国家自然科学基金 81973956:国家自然科学基金 2018JJ3401:湖南省自然科学基金 19B429:湖南省教育厅科学研究项目 19C1424:湖南省教育厅科学研究项目

本校为第一机构

目的观察电针手厥阴心包经穴对脑缺血后大鼠神经生长抑制因子及其受体表达的影响,探讨电针心包经穴对大鼠脑缺血后神经修复的作用机制。方法将SD大鼠随机分为正常组、假手术组、模型组、心包经组和肺经组,每组18只。颈外动脉插入线栓法制作局灶性脑缺血模型。成模后第2日起电针干预30 min,每日1次,干预6 d休息1 d,分别于7、14、21 d进行取材,每个时间点6只。ELISA检测血清神经生长抑制因子(Nogo-A)含量,RT-qPCR检测脑梗死区Nogo-A及其受体NgR1的mRNA表达。结果与同一时间点正常组和假手术组比较,模型组大鼠血清Nogo-A含量及脑梗死区Nogo-A和NgR1 mRNA表达各时间点均明显升高(P

Objective To observe the effects of electroacupuncture(EA)stimulation of acupoints of pericardium meridian on Nogo-A and NgR1 expression in cerebral ischemia rats;To explore the mechanism of EA at the pericardium meridian on nerve repair after cerebral ischemia in rats.Methods The SD rats were randomly divided into normal control group, sham-operation group, model group, pericardium meridian, and lung meridian group, with 18 rats in each group.The model of focal cerebral ischemia was selected and made by external carotid artery insertion thread embolization method.From the second day after suc...