期刊论文

Identification of Lilii Bulbus and Its Counterfeit by ATR-FTIR and UPLC-DAD

中文

中国中医药信息杂志

1005-5304

2020

27

01

57-62

10.3969/j.issn.1005-5304.201901219

ZYBZH-Y-HUN-24:国家中药标准化项目（中药标准行动计划项目） 201728:国家中医药管理局项目 2019JJ50446:湖南省自然科学基金 kq1801044:长沙市科技计划 :湖南省中药饮片标准化 2018-02-01:功能工程技术研究中心开放基金 2018:湖南中医药大学中药学一流学科

本校为第一机构

目的基于傅里叶变换衰减全反射红外光谱(ATR-FTIR)结合超高效液相色谱-二极管阵列检测器(UPLC-DAD)技术快速区分不同品种百合药材粉末。方法采用ATR-FTIR采集百合样品红外光谱数据并导入Simca软件,建立正交偏最小二乘判别分析(OPLS-DA)模型,在此基础上,联用UPLC-DAD技术测定正品百合、伪品金百合及微波干燥卷丹中王百合苷A含量,分析其差异性。结果OPLS-DA二维得分图及层次聚类分析图对卷丹与百合、卷丹与伪品金百合、热风干燥卷丹与微波干燥卷丹进行了良好的分类。卷丹的王百合苷A含量为2.09~3.28 mg/g,百合的王百合苷A含量为1.36~1.77 mg/g,伪品金百合的王百合苷A含量为1.03~1.05 mg/g,微波干燥...

Objective To quickly distinguish different kinds of Lilii Bulbus powder based on ATR-FTIR and UPLC-DAD. Methods The infrared spectral data of samples were collected by ATR-FTIR, and the data was imported into Simca software to establish the orthogonal partial least squares discriminant analysis (OPLS-DA) model. On this basis, UPLC-DAD was used to determine the content of regaloside A in genuine Lilii Bulbus and Lilium trompeten (counterfeit) and microwave dried Lilium lancifolium Thunb., and the differences among different samples were analyzed...