期刊论文

Effects of Electroacupuncture of Pericardium Meridians Acupoints on SYP and PSD-95 in Serum and Brain Tissue of MCAO Rats

中文

中国中医药信息杂志

1005-5304

2021

28

01

81-85

10.19879/j.cnki.1005-5304.202005293

国家自然科学基金（81704184、81473754）； 湖南省卫健委科研课题（20200739）； 湖南省教育厅科学研究项目（19B429）； 湖南省研究生科研创新项目（CX20190590）；

本校为第一机构

目的观察电针心包经穴对局灶性脑缺血大鼠血清和脑组织突触素(SYP)及突触后致密物-95(PSD-95)表达的影响,探讨针刺对脑缺血后突触可塑性的作用机制。方法将90只SD大鼠随机分为正常组、假手术组、模型组、心包经组、肺经组,每组再分为14、21、28 d 3个时间点,每个时间点6只。采用颈外动脉插入线栓法复制局灶性脑缺血模型(MCAO)。心包经组取\"天泉\"\"曲泽\"\"内关\"\"大陵\",肺经组取\"天府\"\"尺泽\"\"列缺\"\"太渊\",于成模后第2日开始电针治疗,每次30min,连续电针6d,间隔1 d。电针后取材,ELISA检测血清SYP、PSD-95含量,RT-qPCR检测脑组织SYP、PSD-95 mRNA的表达。结果与正常组、假手术组比较,...

Objective To investigate the effects of electroacupuncture of pericardium meridians acupoints on SYP and PSD-95 in serum and brain tissue of MCAO rats; To explore the mechanism of acupuncture on synaptic plasticity after cerebral ischemia. Methods Ninety SD rats were randomly divided into normal group, sham-operation group, model group, pericardial meridians group and lung meridians group. Each group was divided into three time points of 14, 21 and 28 days, with six rats in each time point. The model of focal cerebral ischemia was established b...