期刊论文

Biological Basic Research on Model Rats of Coronary Heart Disease with Blood Stasis Syndrome Based on Urine Metabolomics

中文

中国中医药信息杂志

1005-5304

2021

28

03

63-68

10.19879/j.cnki.1005-5304.202008389

国家自然科学基金（81373551、81603512、81874429）； 湖南省自然科学基金（2019JJ40214）;湖南省自然科学基金青年基金（2019JJ50446）； 湖南省教育厅重点项目（19A380）； 湖南省中医药科研计划重点项目（202004）； 湖南省研究生科研创新项目（CX20190536、CX20190591）；

本校为第一机构

目的研究冠心病血瘀证模型大鼠尿液代谢组学特征,探讨其潜在的生物学基础。方法将20只SD大鼠随机分为假手术组和冠心病血瘀证模型组。采用结扎大鼠左冠状动脉前降支方法制备冠心病血瘀证模型。利用超高效液相色谱-质谱联用技术检测假手术组和冠心病血瘀证模型组尿液样本小分子内源代谢物,进行主成分分析和正交偏最小二乘法-判别分析,筛选潜在生物标志物与显著代谢通路。结果与假手术组比较,冠心病血瘀证模型组有36种差异代谢产物水平紊乱,涉及丙氨酸、天冬氨酸和谷氨酸代谢,磷酸戊糖途径,糖酵解/糖异生,泛酸和辅酶A生物合成,柠檬酸盐循环,丙酮酸代谢,嘌呤代谢,甘油脂代谢,戊糖和葡萄糖醛酸酯的相...

Objectives To study the characteristics of the urine metabolomics of model rats for coronary heart disease blood stasis syndrome, and to explore their potential biological basis. Methods Twenty SD rats were randomly divided into a sham operation group and a coronary heart disease blood stasis syndrome model group. The rats were ligated on the left anterior descending coronary artery to prepare a coronary heart diseases blood stasis model. The UPLC-QE-MS technology was used to detect the endogenous small molecular metabolites in the urine samples of the sham operation group and the coronary hea...