期刊论文

Effects of Yixintai Alcohol Extract on Expressions of Bax and Bcl-2 Protein in Angiotensin Ⅱ-induced Cardiac Fibroblasts in Rabbits

中文

中国中医药信息杂志

1005-5304

2021

28

08

83-86

10.19879/j.cnki.1005-5304.202012520

国家自然科学基金（81673955）； 重大疑难疾病慢性心力衰竭中西医临床协作试点项目（2018年）； 湖南省卫生计生科研计划（20200592、20180710）； 湖南中医药大学中医学一流学科开放基金（2018ZYX43）;湖南中医药大学中医学国内一流建设学科（2018年）； 湖南省科技计划（2018JJ2304）；

本校为第一机构

目的观察益心泰醇提物对血管紧张素Ⅱ(AngⅡ)诱导的兔心肌成纤维细胞(CFs)Bax和Bcl-2蛋白表达的影响,探讨其抗心肌纤维化的作用机制。方法提取新生兔CFs,细胞分为对照组、模型组、氯沙坦钾组和益心泰醇提物低、中、高剂量组。除对照组外,其余各组细胞予AngⅡ处理12 h建立心肌纤维化模型,再加入相应浓度药物培养一定时间,对照组加入不含AngⅡ和药物的培养基培养,CCK-8法检测CFs细胞活力,流式细胞仪检测细胞凋亡率,Western blot检测Bax、Bcl-2蛋白表达。结果与对照组比较,模型组CFs细胞活力明显升高,Bax蛋白表达显著降低,Bcl-2蛋白表达显著升高,细胞凋亡率显著降低(P

Objective To observe the effects of Yixintai alcohol extract on the expressions of Bax and Bcl-2 protein in angiotensin Ⅱ (Ang Ⅱ)-induced cardiac fibroblasts (CFs) in rabbits; To explore the mechanism of anti-myocardial fibrosis of Yixintai alcohol extract. Methods The CFs of newborn rabbits were extracted, and the cells were divided into control group, model group, Losartan potassium group and Yixintai alcohol extract low-, medium-, high-dosage groups. Except for the control group, cells in other groups were treated with Ang Ⅱ for 12 h to e...