期刊论文

Effects of Yixintai on JAK2/STAT3 Signaling Pathway Protein Expression in Rats with Myocardial Ischemia-Reperfusion Injury

中文

中国中医药信息杂志

1005-5304

2021

28

02

54-61

10.19879/j.cnki.1005-5304.202006405

国家自然科学基金（81673955）； 湖南省中医药科研计划项目（201833）； 湖南省教育厅科学研究项目（19C1431）；

本校为第一机构

目的 观察益心泰对心肌缺血再灌注损伤(MIRI)大鼠JAK2/STAT3信号通路相关蛋白表达的影响,探讨其保护心肌的作用机制.方法 采用结扎冠状动脉前降支法建立MIRI大鼠模型.实验大鼠随机分为假手术组、模型组和益心泰低、中、高剂量组.TTC染色检测心肌梗死面积,TUNEL法检测细胞凋亡,ELISA检测白细胞介素(IL)-6、IL-1β和肿瘤坏死因子-α(TNF-α)水平,Western blot检测Bax、Bcl-2、Caspase-3、LC3、Beclin 1、p62、JAK2、p-JAK2、STAT3及p-STAT3蛋白表达.结果 与假手术组比较,模型组大鼠肌酸激酶同工酶(CK-MB)和心肌肌钙蛋白Ⅰ(cTnⅠ)表达明显升高(P

Objective To observe the effects of Yixintai on JAK2/STAT3 signaling pathway protein expression in rats with myocardial ischemia-reperfusion injury(MIRI);To explore its myocardial protective mechanism.Methods A rat model of MIRI was established by ligating the anterior descending coronary artery.Experimental rats were randomly divided into sham-operation group, model group and Yixintai low-, medium-and high-dosage groups.TTC staining was used to detect the area of myocardial infarction;TUNEL method was used to detect cell apoptosis;ELISA was us...