期刊论文

Effects of Endothelial Progenitor Cells Induced by Xuefu Zhuyu Decoction on Angiogenesis of Ischemic Region in Rats with Myocardial Ischemia

中文

中国中医药信息杂志

1005-5304

2021

28

02

69-75

10.19879/j.cnki.1005-5304.202006290

国家自然科学基金（81574039、81503627）；

本校为第一机构

目的观察血府逐瘀汤诱导内皮祖细胞(EPCs)对心肌缺血模型大鼠缺血区血管新生的影响,探讨其作用机制。方法采用结扎冠状动脉左前降支方法制备急性心肌缺血模型。实验大鼠随机分为正常组、假手术组、模型组、他汀组和血府逐瘀汤低、中、高剂量组。造模后将荧光标记的EPCs尾静脉注射植入模型大鼠体内,成模后灌胃给药,给药第3日和第7日分别采血,取材缺血坏死部位肉芽组织和肌肉组织,行冰冻切片分析荧光表达,HE染色观察样本坏死病变情况,免疫组化检测心肌组织CD106、CD146的表达,计数新生血管数量,硝酸还原酶法检测血清和心肌缺血区一氧化氮(NO)表达。结果与模型组比较,血府逐瘀汤各剂量组均能促进EP...

Objective To observe the effects of endothelial progenitor cells (EPCs)induced by Xuefu Zhuyu Decoction on angiogenesis in ischemic region of rats with myocardial ischemia;To explore its mechanism.Methods Acute myocardial ischemia model was prepared by ligating the left anterior descending coronary artery.Experimental rats were randomly divided into normal group, sham-operation group, model group, atorvastatin group, Xuefu Zhuyu Decoction low-, medium-, and high-dosage groups.After successful modeling, fluorescently labeled EPCs were injected into the model mice by tail vein injection.After mo...