期刊论文

Content Determination of Three Active Components in Danggui Shaoyao Powder Extracts and Its Drug-containing Serum by HPLC-MS/MS

中文

中国中医药信息杂志

1005-5304

2021

28

01

92-99

10.19879/j.cnki.1005-5304.202005099

国家自然科学基金（81774129、82004184）； 湖南省自然科学基金面上项目（2018JJ2296）;湖南省自然科学基金青年基金（2019JJ50441）； 湖南省高校创新平台开放基金项目（19K065）； 湖南省教育厅科学研究项目（18B246）； 湖南创新型省份建设专项（2019RS1064）；

本校为第一机构

目的建立测定当归芍药散提取物和含药血清中3种活性成分含量的高效液相色谱串联质谱方法。方法药物提取物样品经甲醇-水（1∶1,V/V）溶液溶解、离心、净化后过0.45μm滤膜,血清样品适当稀释、过膜后用高效液相色谱串联质谱法测定。色谱柱为Agilent Poroshell 120 EC-C18（100 mm×2.1 mm,2.7μm）,茯苓酸流动相为乙腈-0.5mmol/L乙酸铵水溶液（9∶1,V/V）,阿魏酸和芍药苷以乙腈-5mmol/L乙酸铵水溶液为流动相梯度洗脱,流速0.4 mL/min,柱温35℃,进样量5μL,采用电喷雾离子源,负离子模式,干燥气温度350℃,干燥气流速12L/min,检测方式为多反应监测。结果当归芍药散中阿魏酸、芍药苷和茯苓酸分别在0.1...

Objective To establish a method for the determination of three active components in Danggui Shaoyao Powder extracts and its drug-containing serum by HPLC-MS/MS. Methods The extract samples were dissolved in methanol-water (1:1, V/V) solution, centrifuged and purified, and then passed through a 0.45 μm filter membrane. Serum samples were appropriately diluted, passed through the membrane and determined by HPLC-MS/MS. The column was Agilent Poroshell 120 EC-C_(18) (100 mm ×2.1 mm, 2.7 μm). The mobile phase of porcine acid was acetonitrile- 0.5...