期刊论文

Effects of Yixintai Alcohol Extract on Expressions of TGF-β1/Smad3 Signaling Pathway Related Factors in Rabbits with Adriamycin-induced Chronic Heart Failure

中文

中国中医药信息杂志

1005-5304

2021

28

12

45-49

10.19879/j.cnki.1005-5304.202104257

81673955:国家自然科学基金 2018:重大疑难疾病慢性心力衰竭中西医临床协作试点项目 20200592:湖南省卫生计生科研计划项目 C20180710:湖南省卫生计生科研计划项目 2018:湖南中医药大学中医学国内一流建设学科 2018JJ2304:湖南省科技计划 2021064:湖南省中医药科研项目

本校为第一机构

目的 观察益心泰醇提物对阿霉素致慢性心力衰竭(CHF)兔TGF-β1/Smad3信号通路相关因子表达的影响,探讨其作用机制.方法 采用阿霉素耳缘静脉注射建立CHF兔模型,将成模兔随机分为对照组、模型组、氯沙坦钾组(2.73 mg/kg)及益心泰醇提物低、中、高剂量组(1.77、3.54、7.08 g/kg),分别给予相应药物灌胃,模型组和对照组予等体积蒸馏水灌胃,1次/d,连续4周.超声心动仪检测兔左室射血分数(LVEF);ELISA检测兔血清N末端B型利钠肽原(NT-proBNP)、血管紧张素Ⅱ(AngⅡ)含量;TUNEL染色法检测兔心肌细胞凋亡率;Western blot检测兔心肌组织Bax、Bcl-2、转化生子因子β1(TGF-β1)、Smad3、Ⅰ型胶原(CollagenⅠ)、...

Objective To explore the effects of Yixintai alcohol extract on expressions of TGF-β1/Smad3 signaling pathway related factors in rabbits with adriamycin-induced chronic heart failure (CHF); To discuss its mechanism of action. Methods A rabbit model of CHF was established by intravenous injection of doxorubicin in the ear. The successful CHF model of rabbits were randomly divided into control group, model group, losartan group (2.73 mg/kg) and Yixintai alcohol extract low-, medium-, high-dosage group (1.77, 3.54, 7.08 g/kg). The corresponding d...