期刊论文

Study on Potential Biomarkers and Metabolic Pathways of Rats in Different Progressive Stages of Coronary Heart Disease with Blood Stasis Syndrome Based on Metabonomics Technology

中文

中国中医药信息杂志

1005-5304

2022

29

01

73-79

10.19879/j.cnki.1005-5304.202104520

国家自然科学基金（81973753）； 湖南省自然科学基金（2018JJ2291）； 研究生质量工程项目（2018CX28、2020CX57）；

本校为第一机构

目的通过超高效液相色谱-飞行时间质谱(UPLC-TOF/MS)的非靶向代谢组学方法探索冠心病血瘀证形成过程中大鼠心肌组织代谢特征,寻找潜在的生物标志物,为基于代谢组学的心肌保护相关治疗措施寻找有意义的靶点。方法取27只SD大鼠作为模型组制备冠心病血瘀证模型,另设空白组8只。模型组大鼠采用高脂饲料喂养+维生素D3灌胃+皮下注射异丙肾上腺素方法分3个阶段进行冠心病血瘀证模型的逐步制备。通过UPLC-TOF/MS技术获取大鼠心肌组织内源性小分子代谢物,利用SIMCA 14.1软件和MetaboAnalyst 5.0软件进行组间差异性代谢物筛选及相关代谢通路分析,使用SPSS21.0对组间差异性代谢物进行定量分析,受试者工作特...

Objective To explore the metabolic characteristics and potential biomarkers of myocardial tissue during the formation of blood stasis syndrome in coronary heart disease through the non-targeted metabolomics method of ultra performance liquid chromatography - time of flight mass spectrometry (UPLC-TOF/MS); To look for potential biomarkers; To find meaningful targets for clinical myocardial protection-related treatment measures based on metabonomics. Methods Twenty-seven SD rats were set as model group to prepare rat models of coronary heart disease with blood stasis syndrome, with 8 rats as bla...