期刊论文

中文

中国中医药信息杂志

1005-5304

2022

29

3

117-123

10.19879/j.cnki.1005-5304.202109557

国家自然科学基金课题（81874375）； 湖南省自然科学基金（2020JJ4464）； 湖南省中医药科研计划重点项目（2021024）； 湖南中医药大学校级科研重点项目（2020XJJJ002）；

本校为第一机构

目的筛选家系早发冠心病血瘀证患者血浆差异蛋白,探讨其对疾病发病的影响,为临床早期诊断提供潜在危险标志物。方法从101例冠心病患者中筛选出典型家系早发冠心病血瘀证患者、家系早发冠心病非血瘀证患者各3例并匹配健康对照组2例,采用同位素标记相对与绝对定量(iTRAQ)技术检测各组血浆蛋白表达情况,通过组间比较分析得出家系早发冠心病血瘀证血浆差异蛋白谱,根据差异蛋白特性及疾病病理特点筛选出疾病早期诊断的潜在危险标志物,以Western blot验证筛选出的蛋白。结果通过iTRAQ技术共得到差异蛋白84个,其中家系早发冠心病血瘀证组与健康对照组之间差异蛋白共32个,包括上调蛋白22个、下调蛋白10个...

Objective To screen the plasma differential proteins of pedigree in premature coronary heart disease(PCHD)patients with blood stasis syndrome(BSS),and to explore their effects on the pathogenesis of the disease,so as to provide potential risk markers for early clinical diagnosis.Methods From 101 cases of coronary heart disease,3 cases of pedigree in PCHD with BSS group and 3 cases of pedigree in PCHD with non-BSS group were selected,and matched with 2 cases of healthy control group.The expression of plasma protein in each group was detected by isobaric tags for relative and absolute quantifica...