期刊论文

中文

中国中医药信息杂志

1005-5304

2022

29

8

9-17

10.19879/j.cnki.1005-5304.202203153

国家自然科学基金（U20A20408、82074450）； 湖南省自然科学基金（2020JJ4066）； 湖南中医药大学中西医结合一流学科开放基金（2020ZXIJH35）； 湖南省研究生科研创新项目（QL20210173）； 广州市卫生健康科技项目（20221A011102）；

本校为第一机构

目的 探究下瘀血汤的活性成分及其抗肝癌的作用机制.方法 应用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS)分析鉴定下瘀血汤的活性成分,借助TCMSP、PubChem、SwissTargetPrediction、UniProt数据库收集活性成分对应靶点.通过GeneCards、OMIM数据库筛选原发性肝癌的相关靶点,采用Cytoscape3.8.2构建成分-靶点-疾病网络.借助STRING数据库构建蛋白相互作用网络,运用Metascape数据库对靶点进行GO功能及KEGG通路富集分析.利用分子对接验证下瘀血汤主要活性成分与核心靶点的相互作用.采用CCK8法检测下瘀血汤含药血清对肝癌细胞HepG2和Huh-7增殖的影响,并通过RT-qPCR验证其对关键靶点的作用.结...

Objective To explore the active components of Xiayuxue Decoction and its anti-liver cancer mechanism.Methods The active components of Xiayuxue Decoction were analyzed by UPLC-Q-TOF-MS.Targets corresponding to active components were collected with the help of TCMSP,PubChem,SwissTargetPrediction,and UniProt databases.The related targets of primary liver cancer were screened through GeneCards and OMIM databases,and the component-target-disease network was constructed by Cytoscape 3.8.2.The PPI network was constructed with the STRING database,and the GO function and KEGG pathway enrichment analysi...