期刊论文

Effects of Danshen Tongluo Jiedu Decoction Combined with METTL3 on Angiogenesis of Hypoxia/reoxygenation Myocardial Microvascular Endothelial Cells of Rats

中文

中国中医药信息杂志

1005-5304

2023

30

05

71-76

10.19879/j.cnki.1005-5304.202209532

82074392:国家自然科学基金 2019JJ40210:湖南省自然科学基金项目

本校为第一机构

目的探讨丹参通络解毒汤联合甲基转移酶样3(METTL3)对缺氧/复氧(H/R)大鼠心肌微血管内皮细胞(CMECs)血管新生的影响及可能作用机制。方法建立H/R损伤CMECs模型,将细胞分为空白组、模型组、沉默组、过表达组...展开更多 目的探讨丹参通络解毒汤联合甲基转移酶样3(METTL3)对缺氧/复氧(H/R)大鼠心肌微血管内皮细胞(CMECs)血管新生的影响及可能作用机制。方法建立H/R损伤CMECs模型,将细胞分为空白组、模型组、沉默组、过表达组、中药+沉默组、中药+过表达组,细胞划痕实验观察CMECs迁移情况,CCK-8法检测CMECs增殖能力,RT-PCR检测CMECs血管内皮生长因子(VEGF)mRNA表达,Western blot检测内皮型一氧化氮...

Objective To investigate the effects and possible mechanism of Danshen Tongluo Jiedu Decoction combined with methyltransferase-like 3(METTL3)on angiogenesis of hypoxia/reoxygenation(H/R)myocardial microvascular endothelial cells(CMECs)of rats.Methods H/R injury CMECs mo...MORE Objective To investigate the effects and possible mechanism of Danshen Tongluo Jiedu Decoction combined with methyltransferase-like 3(METTL3)on angiogenesis of hypoxia/reoxygenation(H/R)myocardial microvascular endothelial cells(CMECs)of rats.Methods H/R injury CMECs model was established,the cells were divided into blan...