期刊论文

中文

中国中医药信息杂志

1005-5304

2024

31

01

110-116

10.19879/j.cnki.1005-5304.202302103

82074392:国家自然科学基金 2019JJ40210:湖南省自然科学基金项目 A2023013:湖南省中医药科研课题 2023JJ50036:湖南省自然科学基金项目

本校为第一机构

目的 研究丹参通络解毒汤含药血清调控转移相关肺腺癌转录本1（MALAT1）对缺氧/复氧大鼠心肌微血管内皮细胞（CMECs）的保护作用及机制。方法 体外培养大鼠CMECs，构建缺氧/复氧损伤细胞模型，转染过表达/沉默空白MALAT1慢病毒，将细胞分为过表达空白+中药组、过表达MALAT1+中药组、过表达MALAT1组、沉默空白+中药组、沉默MALAT1组、沉默MALAT1+中药组，分别予相应血清培养，免疫荧光染色检测Beclin-1蛋白表达，Western blot检测丝氨酸/苏氨酸蛋白激酶1（SRPK1）、丝氨酸丰富剪接因子1（SRSF1）、血管内皮生长因子（VEGF）及Bax蛋白表达，RT-PCR检测MALAT1、SRPK1、SRSF1mRNA表达。结果 与过表达...

Objective To investigate the protective effect and mechanism of Danshen Tongluo Jiedu Decoction medicated serum for hypoxia/reoxygenation rat myocardial microvascular endothelial cells(CMECs)by regulating MALAT1.Methods Rats CMECs cells were cultured in vitro to establish a model of hypoxia/reoxygenation damaged cells,and were transfected overexpressing/silencing blank MALAT1 slow virus,cells were divided into overexpressed blank + TCM group,overexpressed MALAT1 + TCM group,overexpressed MALAT1 group,silenced blank + TCM group,silenced MALAT1 group,and silenced MALAT1 + TCM group.They were cul...