期刊论文

中文

中华中医药杂志

1673-1727

2014

29

3

745-748

国家自然科学基金面上项目（No.30973771）； 教育部博士点基金博士导师项目（No.20094323110004）~~；

本校为第一机构

目的:检测寿胎丸对复发性流产模型小鼠子宫蜕膜组织热休克蛋白27(HSP27)及转铁蛋白(Tf)表达的影响,探讨寿胎丸可能作用的环节.方法:建立复发性流产模型,用双相电泳和质谱技术等蛋白质组学技术,寻找出与复发性流产相关的差异蛋白质HSP27、Tf,用Western Blot和免疫组化实验检测寿胎丸对HSP27和Tf蛋白表达的影响.结果:Western Blot和免疫组化结果显示,小鼠模型组与正常组相比,蜕膜HSP27表达明显升高,Tf表达显著下降,差异有统计学意义(P＜0.01);寿胎丸高、中、低剂量组与模型组相比,蜕膜HSP27表达均降低,差异有统计学意义(P＜0.01),寿胎丸高剂量组与中、低剂量组相比差异有统计学意义(P＜ 0.01).寿胎...

Objective： To detect the effective mechanisms of Shoutaiwan on the expression of uterus decidual tissues HSP27 and Tf in recurrent spontaneous abortion model mice, and to explore to the possible role of the link. Methods： The preparation of recurrent abortion models, using two dimensional gel electrophoresis and mass specu＇ometry proteomics technology, to find out the differences in protein associated with recurrent spontaneous abortion of HSP27 and Tf. The expression effect of HSP27 and Tf with Sboutaiwan was detected by immunohistochemical...