期刊论文

中文

中华中医药杂志

1673-1727

2015

30

8

2975-2979

(No.30873273.No.81273769):国家自然科学基金项目 (No.2011FJ3141):湖南省科技厅科技计划项目 (No.30873273.No.81273769):National Natural Science Foundation of China (No.2011FJ3141):Science and Technology Project of Hunan Provincial Science and Technology Department

本校为第一机构

目的:筛选寻常型银屑病特异性miRNA并分析其靶基因及基因功能,同时观察中药竹黄颗粒剂的干预作用。方法:采集银屑病组和正常组皮肤,运用miRNA基因芯片检测两组miRNA的表达谱,分析得出差异表达的miRNA,并预测其靶基因及进行功能富集分析。同时分析竹黄颗粒剂干预前后患者皮损miRNAs的表达差异。结果:银屑病组有55个明显上调的miRNA,12个下调miRNA,其中5个特异性miRNAs靶基因预测得出AIRE等33个关键基因;功能分析得出其调控了MAPKs等30项主要信号通路;竹黄颗粒剂对银屑病皮损中miR-192等11个差异miRNA有显著影响。结论:miR-31等67个特异性miRNA可能通过影响信号通路参与了银屑病的发病,其有望成为...

Objective： Screening of psoriasis vulgaris specific miRNAs, investigating their target genes and gene function and observing the intervention of Zhuhuang Granule. Methods： Skin samples were collected from the psoriasis group and the normal group. The miRNA microarray technique was used to detect the expression profiles of two groups of miRNA. The obtained differentially expressed miRNA were analyzed. We predict the target genes and their functional enrichment analysis has been done. The effect of Zhuhuang Granule before and after the interven...