期刊论文

中文

中华中医药杂志

1673-1727

2015

30

6

2094-2096

国家自然科学基金项目（No.30801496） 湖南省教育厅重点资助项目（No.10A089） 湖南省自然科学基金重点资助项目（No.13JJ2032）

本校为第一机构

目的:探讨液氮快速冻融法水蛭提取物(HE)对人肝癌Hep G2细胞的化疗药物敏感性影响及诱导凋亡的机制。方法:以5-氟尿嘧啶(5-FU)、阿霉素(ADM)为对照,MTT法检测HE与5-FU或AMD联合用药对人肝癌Hep G2细胞的增殖抑制作用,流式细胞术测定细胞凋亡;采用逆转录聚合酶联反应(RT-PCR)法、Western blot法检测Hep G2细胞经HE处理后多药耐药基(MDR1)、Bax、Caspase3基因的m RNA及蛋白表达变化。结果:HE可增强5-FU、ADM对Hep G2细胞的增殖抑制作用及诱导细胞凋亡(P

Objective： To study the effects and mechanism of himdo extract （HE） on the sensitivity of chemotherapeutic drugs in human hepatoma HepG2 cells. Methods： HepG2 cells were treated in vitro by HE combined with 5-FU or ADM as the control groups. The growth suppression and apoptosis were examined by MTT and flow cytometry assay respectively. Expressions of mRNA and protein of MDR1, Bax and Caspase3 were detected by RT-PCR and Western blot methods in HepG2 cells treated by HE. Results： HE enhanced the suppression of HepG2 cells proliferation cau...