期刊论文

中文

中华中医药杂志

1673-1727

2016

31

4

1398-1401

国家自然科学基金项目（No.81373578,No.81403387,No.81403379） 湖南省中医药管理局项目（No.201594）

本校为第一机构

目的:考察百事乐加味方对脑卒中后抑郁症(PSD)大鼠海马、前额叶皮层病理形态及脑源性神经影响因子(BDNF)水平的影响。方法:采用大脑中动脉线栓(MCAO)法建立大鼠局灶性脑缺血模型,再结合慢性不可预见的温和性应激(CUMS)和孤养法建立PSD,通过HE染色和免疫组化技术,观察百事乐加味方对PSD大鼠海马、前额叶皮层形态及BDNF的影响。结果:HE染色显示:百事乐加味方组大鼠海马CA1区神经元排列稍不整齐,大部分神经元细胞核、核膜结构较清楚, 部分细胞尼氏体消失,少部分细胞胞浆浓缩深染, 可见少部分变性或坏死的细胞, 损伤程度较PSD组轻;百事乐加味方组大鼠前额叶皮层细胞形态未发生明显改变,结构部分清晰...

Objective: To investigate the effects of the modified Baishile Formula on the morphology of hippocampusprefrontal cortex circuit and the level of BDNF in the rat with PSD model. Methods: PSD mode1 was established with chronic unpredictable mild stress (CUMS) on the basis of focal cerebral ischemia model that induced by the method of middle cerebral artery occlusion (MCAO). HE staining technology was used to observe the changes in the morphology of hippocampus and prefrontal cortex, and the immunohistochemistry technology was used to detect the expression of BDNF. Results: HE staining showed th...