期刊论文

Effects of Buyang Huanwu Decoction serum on the expression of Notch signaling pathway related factors mRNA in neural stem cells after hypoxia injure in vitro

中文

中华中医药杂志

1673-1727

2016

31

11

4515-4519

国家自然科学基金项目（No.81273989）~~；

本校为第一机构

目的:探讨补阳还五汤含药血清对体外缺氧损伤神经干细胞(NSCs)增殖及Notch信号通路相关因子mRNA表达的影响。方法:体外分离培养小鼠胚胎NSCs,取第3代悬浮生长的NSCs随机分组,空白组予以无血清条件培养基常氧培养,其余3组分别给予缺氧4h、缺氧4h加10%正常血清和缺氧4h加10%补阳还五汤含药血清干预;培养第5天观察细胞增殖情况,同时提取细胞总RNA,Real-time PCR检测Notch通路4个关键节点因子Notch-1、 Hes-1、Neurogenin-1(Ngn-1)和Delta-like-1(Dll-1)mRNA相对表达水平。结果:正常生长的NSCs Notch-1、Hes- 1 mRNA高表达,Ngn-1、Dll-1 mRNA低表达;缺氧4h后NSCs克隆球数减少(P

Objective: To investigate the effects of Buyang Huanwu Decoction serum on the expression of Notch signaling pathway related factors mRNA in neural stem cells (NSCs) after hypoxia injure in vitro. Methods: The embryos NSCs were isolated from fetal mouse brain and cultured in vitro, and the third generation of suspended growth of the neural spheres were randomly divided into four groups. NSCs of blank group were treated with serum free conditioned culture medium and normal oxygen, and the other three groups were given with hypoxia for 4h, hypoxia for 4h and 10% normal serum intervention as well ...