期刊论文

Effects of Buyang Huanwu Decoction on brain tissue pathologic morphology and expression of NO, vWF and 6-keto-PGF1α in rat model of MCAO at different time points

中文

中华中医药杂志

1673-1727

2016

31

12

4987-4991

国家自然科学基金

本校为第一机构

目的:探讨补阳还五汤对中脑动脉闭塞(MCAO)后局灶性脑缺血大鼠不同时间点脑组织病理形态及一氧化氮(NO)、血管性假血友病因子(vWF)、6-酮-前列腺素F1α(6-keto-PGF1α)表达的影响。方法:用颈内动脉线栓法建立MCAO模型,采用HE染色、TUNEL法观察缺血后1d、3d、7d脑组织病理形态;采用酶联免疫吸附法(ELISA)测定血浆中NO、vWF、6-keto-PGF1α的表达。结果:与模型组同期比较,补阳还五汤组脑组织梗死范围在治疗后7d减少最明显(P

Objective: To explore the effects of Buyang Huanwu Decoction (BHD) on brain tissue pathologic morphology and expression of nitric oxide (NO), von willebrand factor (vWF) and 6- keto prostaglandin 1α (6-keto-PGF1α) in rat model of MCAO at different time points. Methods: The rat model of MCAO was established by the method of nylon monofilament via the internal carotid artery. The pathologic morphology of brain tissues after ischemia in day 1, day 3 and day 7 were observed by hematoxylin-eosinstaining (HE) and transferase-mediated deoxyuridine t...