期刊论文

Effects of Pediatric Chronic Cough Mixture on the content of SP and the expressions of SP, VR1 and PGP-9.5 in lung tissue of rats with post infectious cough with lung yin deficiency syndrome

中文

中华中医药杂志

1673-1727

2018

33

12

5615-5619

湖南省发展和改革委员会基金项目(No.湘财企指[2015]83) 湖南省教育厅优秀青年基金项目(No.15B171) 湖南省自然科学基金项目(No.2015JJ2016).

本校为第一机构

目的:观察小儿久咳合剂对感染后咳嗽(肺热阴虚证)大鼠肺匀浆中P物质(SP)含量及肺组织中SP、类香草素受体1(VR1)及蛋白基因产物9.5(PGP-9.5)表达的影响,探讨该药对大鼠气道神经源性炎症的调节作用。方法:从50只SD大鼠中随机选取10只作为空白组,其余40只采用烟熏+内毒素滴鼻+甲状腺素灌胃+辣椒素雾化诱咳法建立感染后咳嗽(肺热阴虚证)大鼠模型,造模成功后随机分为模型组、孟鲁司特钠组、小儿久咳合剂小剂量组、小儿久咳合剂大剂量组,每组10只。各组分别予以相应药物进行干预,空白组及模型组则给予同体积0.9%氯化钠溶液灌胃。ELISA法检测治疗前后各组大鼠肺匀浆中SP含量,免疫组化法检测治疗前后大鼠...

Objective: To observe the effects of Pediatric Chronic Cough Mixture (PCCM) on the content of SP and the expressions of SP, VR1, PGP-9.5 in lung tissue of rats with post infectious cough (PIC) with lung yin deficiency syndrome, and to explore its regulating action on airway neurogenic inflammation. Methods: The fifty rats were enrolled and randomly divided into control group (n=10) and model group (n=40). The PIC (lung yin deficiency syndrome) model rats were made by cigarettesmoking exposure, intranasal administration with small dose of LPS, t...