期刊论文

Effects of Zhibai Dihuang Decoction on sperm MMP, ROS and CytC in rats infected with ureaplasma urealyticum

中文

中华中医药杂志

1673-1727

2018

33

9

3879-3882

No.81603634:国家自然科学基金 No.81774324:国家自然科学基金 No.2015101:湖南省中医药局项目

本校为第一机构

目的:研究知柏地黄汤对解脲脲原体(U U)感染模型大鼠精子线粒体膜电位(M M P)、活性氧(ROS)水平及细胞色素C(CytC)含量的影响.方法:雄性SD大鼠80只,随机分成假手术组、模型组、中药组(知柏地黄汤,2mL/d)、中西组(知柏地黄汤+强力霉素)、西药组(强力霉素,2mL/d),除假手术组外,其他组均采用经大鼠膀胱注射UU建立UU感染动物模型.连续灌胃给药21d后处死取标本检测,采用荧光流式细胞技术(JC-1染色)测定精子MMP水平(JC-1+%)及ROS水平(JC-1+%),采用ELISA法检测CytC含量.结果:模型组大鼠精子MMP(JC-1+%)、ROS水平(JC-1+%)及精子中CytC含量,与假手术组比较,差异显著(P

Objective: To study the effect of Zhibai Dihuang Decoction (ZDD) on the mitochondrial membrane potential (MMP), reactive oxygen species (ROS) level and cytochrome C (CytC) content in the sperm of ureaplasma urealyticum (UU) infection model rats. Methods: A total of 80 male SD rats were randomly divided into the sham operation group, the model group, the Chinese materia medica group (ZDD, 2mL/d), the combined Chinese materia medica and western medicine group (ZDD+Doxycycline), and western medicine group (Doxycycline, 2mL/d). In addition to the sham operation group, UU infection models were esta...