期刊论文

Study on chemical composition of volatile oil from Taraxaci Herba and its antiinflammatory and anti-tumor activities

中文

中华中医药杂志

1673-1727

2018

33

7

3106-3111

湖南省自然科学基金项目

本校为其他机构

目的:研究蒲公英挥发油的化学成分组成以及抗炎抗肿瘤活性,为进一步开发利用蒲公英提供实验依据.方法:采用超临界流体CO_2萃取法对蒲公英挥发油进行提取,通过GC-MS联用仪对挥发油进行成分分析,利用脂多糖(LPS)诱导炎症模型研究挥发油抗炎活性;采用乳腺癌细胞(MCF-7)、小鼠乳腺癌(4T1)组织 HE、TUNEL染色和Micro-CT扫描研究蒲公英挥发油抗乳腺癌活性.结果:超临界流体CO_2萃取法提取蒲公英挥发油,萃取率为5.55%;GC-MS联用分析鉴定蒲公英挥发油成分26个,占总挥发油成分83.72%,主要成分为亚麻酸(51.70%)、棕榈酸(18.41%)、油酸(2.75%)、棕榈酸乙酯(1.94%)、二十二烷酸(1.40%)、十九烷酸(1.23%)等,其...

Objective: To investigate the chemical composition of volatile oil from Taraxaci Herba and the antiinflammatory and anti-tumor activities in vitro, and to provide experimental basis for further development of Taraxaci Herba. Methods: The volatile oil from Taraxaci Herba was extracted and analyzed by supercritical fluid CO_2 extraction technique and GCMS, then the anti-inflammatory activity in vitro was studied by using the lipopolysaccharide (LPS) to induce inflammation model. The anti-tumor activity of volatile oil was studied by the HE, TUNEL...