期刊论文

Study of oxymatrine on inhibiting proliferation and inducing apoptosis of nasopharyngeal carcinoma CNE2 cells through MAPK/ERK pathway

中文

中华中医药杂志

1673-1727

2019

34

9

4306-4309

No.81573721:国家自然科学基金 No.81874408:国家自然科学基金 No.2016JJ6117:湖南省自然科学基金 No.2019JJ40216:湖南省自然科学基金 No.YF201717:创新项目 No.2018404:湖南省大学生创新项目

本校为第一机构

目的:研究氧化苦参碱(OMT)对鼻咽癌CNE2细胞增殖和凋亡的影响及作用机制。方法:采用MTT法检测OMT对CNE2细胞增殖的影响;流式细胞术测细胞凋亡率;Western Blot法检测增殖、凋亡相关蛋白及MAPK/ERK信号通路关键蛋白的表达水平。结果:OMT对鼻咽癌CNE2细胞增殖的抑制率随时间(24、48、72h)和药物浓度(5、10、20、40、80μmol/L)的增加而增加。与溶剂对照组比较,OMT能诱导CNE2细胞凋亡(P

Objective: To study the effect and mechanism of oxymatrine(OMT) on proliferation and apoptosis of nasopharyngeal carcinoma CNE2 cells. Methods: MTT method was used to detect the effect of OMT on CNE2 cells proliferation. Apoptosis rate was measured by flow cytometry. Western Blot was used to detect the expression levels of proliferation-related proteins, apoptosis-related proteins and key proteins of MAPK/ERK signaling pathway. Results: The inhibitory effect of oxymatrine on the proliferation of nasopharyngeal carcinoma cells increased with the...