期刊论文

Study on the mechanism of Sini Decoction against atherosclerosis based on TLR-2/4-IRF3 signaling pathway

中文

中华中医药杂志

1673-1727

2019

34

11

5470-5474

国家自然科学基金面上项目(No.81373716) 湖南省中医药管理局项目(No.201518) 湖南省研究生创新课题(No.CX2017B429)。

本校为第一机构

目的:从TLR-2/4-IRF3信号传导通路的角度,观察四逆汤对动脉粥样硬化(AS)的影响,从蛋白分子水平探讨四逆汤促胆固醇逆转运抗AS形成的作用机制。方法:将60只AS模型家兔随机分为空白对照组,模型组,阿托伐他汀组,四逆汤低、中、高剂量组,观察各组家兔主动脉形态,比较各组家兔治疗后血清中TC、TG、 LDL-C、HDL-C、ApoA1、Apo-B水平的变化,以及腹主动脉TLR-2、TLR-4、IRF3和ABCA1蛋白表达变化,并在ox-LDL诱导的巨噬细胞上检测四逆汤对TLR-2、TLR-4、IRF3和ABCA1表达水平的干预作用。结果:阿托伐他汀组、四逆汤组对泡沫细胞的形成、VSMCS增殖都有抑制作用。与空白对照组比较,模型组血清TC、TG、LDL-C、...

Objective: To observe the effects of Sini Decoction on atherosclerosis (AS) through TLR-2/4-IRF3 signaling pathway, and to investigate its mechanism in anti-AS by promoting the reverse cholesterol transport. Methods: Sixty rabbits were divided randomly into 6 groups including control group, AS group without treatment, AS group with atorvastatin treatment, AS group with low-dose Sini decoction treatment, AS group with medium-dose Sini decoction treatment, and AS group with high-dose Sini decoction treatment. HE staining was used for the aortic morphologies. The levels of TC, TG, LDL-C, HDL-C, A...