期刊论文

Analysis of the metabolomics characteristics in Zusanli (ST36) area of CAG model rats based on meridian-viscera

中文

中华中医药杂志

1673-1727

2019

34

2

768-772

No.2015CB554502:国家重点基础研究发展计划（973计划） No.81202770:国家自然科学基金 No.81574082:国家自然科学基金 No.81603705:国家自然科学基金

本校为第一机构

目的:建立并验证慢性萎缩性胃炎(CAG)大鼠模型,运用代谢组学的方法研究CAG大鼠模型代谢组学特征及生物标志物,探讨胃经穴位与胃之间是否存在一定的相对特异性联系。方法:将32只SD大鼠随机分为空白组和模型组,通过MNNG联合乙醇灌胃、饥饱失常法复制CAG大鼠模型。HE染色观察大鼠胃组织病理学变化,采用1H-NMR检测足三里穴区及胃组织代谢物并分析所得数据。结果:造模结束后,模型组大鼠胃黏膜色苍白,黏膜层变薄,弹性减少,皱襞变平,黏液减少,有散在出血点,HE染色结果可见大量炎性细胞浸润,大量主细胞坏死空泡样变化,大量纤维组织充填,并有诸多集中出血点,腺体结构紊乱减少,固有腺体出现萎缩。与空白组...

Objective: To establish and validate a rat model of chronic atrophic gastritis (CAG), metabolomics is applied to study the metabolomics characteristics and biomarkers of CAG rat model, and to explore whether there is a certain relative specificity relationship between gastric acupoints and stomach. Methods: Thirty-two Sprague-Dawley rats were randomly divided into blank group and model group. The CAG rat model was replicated by MNNG combined with ethanol gavage and abnormal of starvation. The pathological changes of gastric tissue were observed by HE staining. The metabolites of Zusanli (ST36)...