期刊论文

Study on borneol combined with astragaloside IV and panax notoginseng saponins promoting nerve repair after cerebral ischemia-reperfusion

中文

中华中医药杂志

1673-1727

2019

34

12

5854-5859

国家自然科学基金项目(No.81573875) 湖南省中医药管理局重点项目(No.201509) 湖南省科技厅科技创新平台与人才计划-中医脑病临床研究中心(No.2017SK4005) 2017年度湖南省大学生研究性学习和创新性实验计划项目(No.1029) 湖南中医药大学“十三五”一级学科基础医学建设项目(No.6).

本校为第一机构

目的:探讨冰片、黄芪甲苷(ASTⅣ)和三七总皂苷(PNS)配伍促进脑缺血再灌注后神经修复的作用和Wnt/β-catenin信号机制。方法:采用大鼠局灶性脑缺血再灌注模型,灌胃给药冰片、ASTⅣ、PNS及其配伍药物,神经功能评分和病理形态评价药物对脑组织损伤的影响,免疫组织化学方法检测脑组织巢蛋白(Nestin)、神经元核抗原(NeuN)反映神经发生,Western blot法检测Wnt/β-catenin信号通路相关蛋白表达。结果:与模型组比较,ASTⅣ+PNS配伍组、冰片+ASTⅣ+PNS配伍高、低剂量组可显著降低神经功能评分(P

Objective: To research the effects of borneol combined with astragalosides Ⅳ (ASTⅣ) and panax notoginseng saponins (PNS) promoting neural repair and the mechanism of Wnt/β-catenin signaling pathway. Methods: Focal cerebral ischemia-reperfusion model of middle cerebral artery in rats was established, borneol, ASTⅣ, PNS and the combinations were administered by gavage, the nerve function score and the neuropathological evaluation were used to assess the influences of the combination on brain injury, the expression of Nestin and neuronal nucle...