期刊论文

Mechanism research of anti influenza virus of ephedra decocted earlier Maxing Shigan Decoction from the expression level of IFN-α/β protein mediated by TLR7/8

中文

中华中医药杂志

1673-1727

2019

34

3

1188-1193

No.81774126:国家自然科学基金 No.81473468:国家自然科学基金 No.81503445:国家自然科学基金 No.81703919:国家自然科学基金 No.17K067:湖南省教育厅创新平台开放基金 No.2016JJ2095:湖南省自然科学基金 No.2017JJ3232:湖南省自然科学基金 No.201701:湖南省中医药科研重点项目 No.15:湖南省高等学校科技创新团队支持计划 No.1:湖南中医药大学重点学科建设项目

本校为第一机构

目的:基于TL R7/8介导的I F N-α/β蛋白表达水平探讨麻黄先煎之麻杏石甘汤抗流感病毒效应机制。方法:以TLR7/8激活剂(R848)、抑制剂(抗体TLR7和抗体TLR8)干预的小鼠巨噬细胞为对照,以酶联免疫吸附技术检测流感病毒干预小鼠巨噬细胞24、48h的IFN-α/β分泌水平。以流感病毒干预的小鼠巨噬细胞为对照,以酶联免疫吸附技术和Western Blot技术检测麻黄先煎之麻杏石甘汤含药血清干预24、48h的流感病毒感染的小鼠巨噬细胞IFN-α/β分泌水平和蛋白表达水平。结果:与正常大鼠血清组及胎牛血清组比较,TLR7/8激活剂(R848)与流感病毒干预24、48h的RAW264.7细胞IFN-α和IFN-β分泌水平和蛋白表达水平显著升高...

Objective: To investigate the anti influenza virus mechanism of ephedra decocted earlier Maxing Shigan Decoction from the expression level of IFN-α/βprotein mediated by TLR7/8. Methods: Mouse macrophages treated with TLR7/8 activator (R848), inhibitor (antibody TLR7 and antibody TLR8) as control, enzyme-linked immunosorbent assay was used to detect influenza virus intervention in mouse macrophages for 24h and 48h IFN-α/βsecretion level. Mouse macrophages treated with influenza virus as control, ephedra decocted earlier Maxing Shigan Decocti...