期刊论文

Effects of Jianpi Yiqi Lishui Fomula on cerebral edema and the expression of Occludin, Claudin-1 and ZO-1 protein in brain of rats with cerebral ischemia

中文

中华中医药杂志

1673-1727

2019

34

11

5424-5428

No.81202632:国家自然科学基金 No.81473567:国家自然科学基金 No.18A208:湖南省教育厅科学研究项目 No.2019JJ40219:湖南省自然科学基金 No.201723:湖南省中医药科研项目

本校为第一机构

目的:探讨健脾益气利水方(J P F)对脑缺血大鼠脑水肿及脑组织紧密连接蛋白闭合蛋白(Occludin)、密封蛋白-1(Claudin-1)、闭锁连接蛋白-1(ZO-1)表达的影响。方法:将105只雄性SD大鼠随机分为假手术组,模型组,依达拉奉组,JPF小、中、大剂量组。采用线栓法制备大脑中动脉栓塞(MCAO)模型,治疗7d后处死,运用伊文思蓝(EB)渗出法检测血脑屏障的通透性,运用免疫组化法检测大鼠缺血侧脑组织Occludin、Claudin-1、ZO-1蛋白表达。结果:与假手术组比较,模型组缺血侧脑组织EB的含量显著增加(P

Objective: To study the effects of Jianpi Yiqi Lishui Fomula (JPF) on brain edema and the expression of tight junction protein Occludin, Claudin-1 and ZO-1 in brain of rats with cerebral ischemia. Methods: A total of 105 male Sprague- Dawley rats were randomly divided into sham-operation group, model group, Edaravone group, JPF low dose group, JPF medium dose group and JPF high dose group. The MCAO model was made by suture method. After 7 days of treatment, the rats were killed. The permeability of blood-brain barrier was measured by evans blue exudation method, and the expression of Occludin,...