期刊论文

Protective effects of Jiawei Naotai Formula on hippocampal neurons induced by hypoxia/reoxygenation based on SIRT1-mediated apoptosis-related signaling pathway

中文

中华中医药杂志

1673-1727

2019

34

10

4516-4522

No.81603604:国家自然科学基金 No.81774174:国家自然科学基金 No.2018M632972:中国博士后科学基金面上项目 No.2019JJ50434:湖南省自然科学基金 No.201822:湖南省中医药科研项目 No.09k056:湖南省教育厅科学研究项目 No.201705:湖南中医药大学大学生研究性学习和创新性实验计划 No.2017XJJJ09:湖南中医药大学校级科研基金项目

本校为第一机构

目的:基于沉默信息调节因子1(SIRT1)介导的凋亡相关信号通路探讨加味脑泰方含药血清对缺氧/复氧(H/R)损伤海马神经元细胞凋亡及Bcl-2、Bax、Caspase-3表达的影响。方法:原代分离、培养及鉴定SD大鼠胎鼠海马神经元,采用缺氧24h、复氧2h处理建立H/R细胞模型,将培养的海马神经元随机分为5组:正常对照组、空白血清对照组、模型组、奥拉西坦含药血清组和加味脑泰方含药血清组,待缺氧24h后,除正常对照组与模型组给予等量培养液外,其余各组分别给予相应体积分数10%的含药血清或空白血清,复氧后继续培养2h;采用MTT法检测细胞活力;采用流式细胞仪检测细胞凋亡率;采用免疫荧光与高内涵细胞成像分析技术(hi...

Objective: To investigate the effect of Jiawei Naotai Formula on apoptosis and expression of Bcl-2, Bax and Caspase-3 in hippocampal neurons induced by hypoxia/reoxygenation based on SIRT1-mediated apoptosis-related signaling pathway. Methods: Hippocampal neurons from SD rats were primitively isolated, cultured and identified. Hypoxia/reoxygenation (H/R) cell model was established by hypoxia 24h and reoxygenation 2h intervention. The cultured cells were randomly divided into normal group, blank serum group, model group, positive drug medicated serum of oxiracetam group and medicated serum of J...