期刊论文

Study on separation and purification process of total alkaloids from Corydalis by macroporous adsorption resin HPD-100

中文

中华中医药杂志

1673-1727

2019

34

6

2754-2757

湖南省长沙市科技局计划课题(No.K1508030-31,No.K1003068-31) 湖南省科技厅计划项目(No.2009SK3125) 湖南省科技厅重点项目(No.2016SK2052) 湖南省教育厅科研项目(No.13c671) 湖南大学生创新课题(No.0110-0901002016,No.1021-0001017096) 湖南省药食同源功能性食品工程技术研究中心开放基金(No.2018YS7Y02) 湖南省中医药管理局科研计划课题重点项目(No.201923).

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目的:优选HPD-100大孔吸附树脂分离纯化延胡索总生物碱的工艺条件,为工业化生产提供依据。方法:采用酸性染料比色法测定延胡索总生物碱的含量,并以此为评价指标,以延胡索醇提浓缩液上样流速、吸附柱径高比、药液浓度3个影响树脂的载样量的因素设计正交试验,对延胡索总生物碱解吸性能进行考察,从而确定延胡索总生物碱解吸剂的类型和用法及洗脱流速。结果:HPD-100大孔吸附树脂有较大吸附量、易吸附、易解吸附,延胡索提取液浓度为0.25g/mL,流速为2BV/h,吸附柱径高比为1∶15,洗脱液体积定为先用60%乙醇5BV再用80%乙醇1BV,洗脱流速为3BV/h,为最优分离纯化工艺条件。结论:该延胡索总生物碱的分离纯化工...

Objective: To optimize the process conditions ofmacroporous adsorption resin HPD-100 for separation and purification of total alkaloids from Corydalis, and to provide basis for industrial production. Methods: The content of total alkaloids in Corydalis was determined by acid dye colorimetry, and it was used as an evaluation index. The orthogonal experiment was carried out with three factors affecting the loading of the resin, such as the loading flow rate of the extract of the fumarate, the diameter ratio of the adsorption column, and the concentration of the liquid. The desorption performance...